Actinometry
Power Measurement in the Lucent360™
To measure the effective irradiation of samples in the Lucent360™, we performed actinometric experiments with several vial configurations. Actinometry is the ideal method to determine how many photons penetrate a reaction vial. The results depend on the geometry of the vial, the reaction volume and the position relative to the light sources. We use a solution of ferrioxalate which is sensitive to light from 365 nm to 475 nm. The Fe3+ is reduced to Fe2+ . The amount of produced Fe2+ is proportional
to the number of photons absorbed by the solution. It is possible to determine the photon flux absorbed by an actinometric solution. You can learn more about actinometry here:
https://www.hepatochem.com/determine-photon-flux-using-actinometry/
In the Lucent360™ we performed several preliminary measurements, and we determined this effective power and photon flux.
See below measurements at 450 nm.
| Volume | Vial | Measured Effective Power | Measured Photon Flux | Light Configuration |
| 2 ml | 4 ml | 1 W | 4×10-6 Einsten/s |  |
| 10 ml | 20 ml | 5 W | 19×10-6 Einsten/s | |
| 100 ml | 700 ml (Reactor) | 40 W | 150×10-6 Einsten/s |  |
For reference the irradiation in a PhotoRedOx Box reaches 0.35W for 2 ml into a 4 ml vial.
More actinometry results will be disclosed in the coming weeks.
