Suzuki Cross Coupling Array Standard Kit (HCK1003-01-001)
1 set of 6 catalysts (Pd(PPh3)4, Pd(
The typical protocol is performed at 0.15 mol/l concentration reaction condition and
Protocol at 100 µl volume reaction condition
requiredvolume of a 0.15 mol/L solution of combined substrates (boronic acid reagent and halogenated substrate) in the selected solvent. For example, 450 µl solution for 4 reaction conditions.
- Using a clean and dry syringe, add 100 µl of substrates solution to a reaction vial and mix with the catalysts using the syringe.
- Add 30 µl of the selected base solution. (2 equivalents)
- Stir the reaction vials in the reaction block at
desiredtemperature (for example:80°C) for 3 to 12 hours.
- Remove the vial caps using a
analyticalsample for each reaction condition with 5 µl sample diluted into 200 µl in either DMSO or water/acetonitrile 50/50. Analyze resulting analytical samples by LC/MS.
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