Buchwald Cross-Coupling HCK1004-01-011

Buchwald Cross Coupling Array:
Pd Amination Strong Base Kit 4

2 sets of 24 reagent combinations in separated vials; 8 Catalysts (BrettPhosG2, RuPhosG2, XPhosG2, tBuXPhosG3, tBuBrettG3, AmphosG3, AdBrettPhosG3 and CataCXium® A G3) and 3 bases (NaOtBu, LHMDS, and P2-Et) 48 reaction vials total.

This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)

The typical protocol is performed at 0.15 mol/l concentration reaction condition and 1 to 1 ratio substrates in 100 µl selected solvent. Resulting catalyst loading is 10%.  Two equivalents of base are preloaded (30 µmol). It is possible to decrease substrate concentration by increasing the reaction volume (see chart below). Maximum volume for reaction condition is 250 µl. Recommended solvents are dioxane, tBuOH or Toluene.

Protocol at 100 µl volume reaction condition

  • Prepare requiredvolume of a 0.15 mol/L solution of combined substrates in the desired sparged solvent. For example, 1900 µl solution for 18 reaction conditions (100 µl extra to compensate potential evaporation).
  • Using a clean and dry syringe, add 100 µl of substrates solution to each reagent vial containing a precatalyst. Stir to dissolve the precatalyst.
  • Transfer content of the precatalyst vial to a reaction vial containing a strong base.
  • Stir the reaction vials in the reaction block at desiredtemperature (for example:80°C) for 3 to 12 hours.
  • Remove the vial caps using a decapper.
  • Prepare analyticalsample for each reaction condition with 5 µl sample diluted into 200 µl in either DMSO or water/acetonitrile 50/50.Analyzeresulting analytical samples by LC/MS.