
Buchwald Cross Coupling Array:
Pd Amination Strong Base Kit 2
(HCK1004-01-005)
2 sets of 24 reagent combinations in separated vials; 8 Catalysts (BrettPhosG2, RuPhosG2, XPhosG2, tBuXPhosG3, tBuBrettG3, Pd2dba3/
This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)
The typical protocol is performed at 0.15 mol/l concentration reaction condition and 1 to 1 ratio substrates in 100 µl selected solvent. Resulting catalyst loading is 10%. Each reaction contains 1.5
Protocol at 100 µl volume reaction condition
- Prepare
required volume of a 0.15 mol/L solution of combined substrates in the desired sparged solvent. (2.7 ml if performing reaction with 3 bases -100 µl extra per base to compensate potential evaporation). - Transfer 900 µl substrate solution to NaOtBu vial and mix.
- Using a clean and dry syringe, add 100 µl of substrates/NaOTBu solution to each catalyst vial A1 through A8 containing a catalyst and a stir bar. Stir to dissolve the catalyst.
- Transfer 900 µl substrate solution to LHDMS vial and mix.
- Using a clean and dry syringe, add 100 µl of substrates/LHDMS solution to each catalyst vial B1 through B8 containing a catalyst and a stir bar. Stir to dissolve the catalyst.
- Transfer 900 µl substrate solution to P2-Et vial and mix.
- Using a clean and dry syringe, add 100 µl of substrates/P2-Et solution to each catalyst vial C1 through C8 containing a catalyst and a stir bar. Stir to dissolve the catalyst.
- Stir the reaction vials in the reaction block at
desired temperature (forexample: 80°C) for 3 to 12 hours. - Remove the vial caps using a
decapper . - Prepare
analytical sample for each reaction condition with 5 µl sample diluted into 200 µl in either DMSO or water/acetonitrile 50/50. Analyze resulting analytical samples by LC/MS.