Amide Coupling Array:
First Choice Kit
(HCK1005-01-007)
2 sets of 7 reagents; DIC, EDC (HCl), HATU, COMU®, PyBOP®, DEPBT, and ethyl(
This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)
The typical protocol is performed at 0.1 mol/l concentration reaction condition using a solution of combined acid and amine.
Protocol at 100 µl volume reaction condition
- Prepare
required volume of a solution of acid substrate 0.2 mol/L in the desired solvent. For example, 350 µl solution for 6 reaction conditions (50 µl extra to compensate potential evaporation). - Using a clean and dry syringe, add 50 µl of the acid solution to each reaction vial containing a coupling reagent. Stir the reaction vials in the reaction block at
desired temperature (between 0°C and 25°C) for 30 min. - Add 10 µl of the solution of ethyl (
hydroxyimino )cyanoacetate to each reaction vial. (prepared by 70 µl of DMF in the reagent vial) - Prepare 350 µl of a solution of amine substrate 0.2 mol/L and base (Et3N or DIPEA) 0.4 mol/L in the desired solvent. Add 50 µl of this solution to each reaction vial.
- Stir the reaction vials in the reaction block at
desired temperature for 3 to 12 hours. Do not heat vials attemperature higher than 120°C or higher than 5°C above the boiling point of the used solvent. - Remove the vial caps using a
decapper . - Prepare
analytical sample for each reaction condition with 5 µl sample diluted into 200 µl in either DMSO or water/acetonitrile 50/50. Analyze resulting analytical samples by LC/MS.
